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. 2021 Apr 9;9:569045. doi: 10.3389/fbioe.2021.569045

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Copyright © 2021 Strasser, Farrell, Ho, Scheffler, Cook, Pankert, Mowlds, Viner, Karger and Bones.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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High vs. low cell culture conditions were compared using volcano plots (A) with cut-off curves indicating significance determined by a two-sided t-test with a permutation-based FDR of 0.05 and S0 of 0.03 (n = 2). Color of the plot indicates spatial density. Significantly regulated proteins were used for hierarchical clustering (B) based on Euclidian distance. Red indicates upregulation, blue shows downregulation of proteins compared to standard conditions. The PCA (C) indicates relationships between prepared technical replicates (n = 2) and applied processing conditions. The Venn-diagram (D) shows the overlap of significantly regulated proteins after shifting temperature, pH, or DO concentration. Data was analyzed using Perseus software (version 1.6.6).