TABLE 1.

Bioprocessing conditions used to prepare CHO DP-12 cell cultures in Sartorius Cultibag Disposable Bioreactors.

Applied bioreactor settings once cells entered stationary phase (9 bioreactors in total)
Parameter	High	Standard	Low
Temperature	High temperature: 39.5°C, pH 7.0, 85% DO	Standard 1: 37.0°C, pH 7.0, 85% DO	Low temperature: 32.0°C, pH 7.0, 85% DO
pH	High pH: 37.0°C, pH 7.2, 85% DO	Standard 2: 37.0°C, pH 7.0, 85% DO	Low pH: 37.0°C, pH 6.8, 85% DO
DO	High DO: 37.0°C, pH 7.0, 110% DO	Standard 3: 37.0°C, pH 7.0, 85% DO	Low DO: 37.0°C, pH 7.0, 60% DO

In total, 9 bioreactors were used resulting in 3 biological replicates for standard conditions. After day 5, cells were cultured under altered conditions for 48 h before harvest.