TABLE 2.

Peptides displaying altered deuterium uptake compared to standard mAb samples analyzed by HDX-MS and related peptide modifications identified by peptide mapping.

Sample	mAb Chain	Peptide sequence	Position	Peptide modification
Low Temp	Light	HWYQQKPGKAPKLLI	H39-I53	Oxidation W40
High pH	Light	AKVQWKVDNALQSGNSQE	A149-E166	Oxidation W153, Deamidation N157, Deamidation Q160, Deamidation Q163
High DO	Heavy	VQSGGGLVQPGGS	V5-S17	n/a
Low DO	Heavy	TLSRDNSKNTAY	T69-Y80	Deamidation N74, Deamidation N77
Low Temp	Heavy	VSWNSGALTSGVHTFPAVL	V161-L179	n/a
Low Temp	Heavy	NSGALTSGVHTFPAVL	N164-L179	n/a
Low Temp	Heavy	TVDKSRWQQGNVFSCSVM	T416-M433	n/a