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. 2021 Apr 9;12:612670. doi: 10.3389/fgene.2021.612670

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Copyright © 2021 Wu, Su and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The relationship between counting efficiency with library size. The log transformed counts are regressed on log library size and the slope is plotted against the correlation between the gene counts and the library size. A slope of 1 indicates a linear effect of library size on counting efficiency. (A) Data from T2D study. (B) Data from hESC study. Only genes with average log counts greater than 4 are included.