FIGURE 6.

When the intracellular cAMP level decreased by washout of BARs/CPT-cAMP, the changes described in Figure 5 reversed in order. Compared to the steady state AP firing (panel A), the AP cycle length is prolonged and LCR ensemble is decreased (panel B), suggesting the disengagement of M- and Ca²⁺-clocks. Shortly after cAMP washout (the panel B), spontaneous AP firing ceased, and the cell returned to dormant state again (panel C) in which the membrane potential drifted around –40 mV, ensemble LCR signal area decreased, and the voltage-Ca²⁺ loop demonstrated only very small fluctuation around one point, rather than crating a loop.