Figure 3.

iMG cells phagocytosed fibrillary Aβ₄₂. Confocal imaging and flow cytometry analysis of iMG cells were incubated with human HiLyte™ Fluor 488-labeled Aβ₄₂ proteins. Phagocytosis activity was measured after 4 h incubation. (A) Representative confocal images were shown where controls cells were panel a (a1–a4) and iMG cells with phagocytosed fAβ₄₂ were panel b (b1–b4). Images were captured at 100× with a scale representing 10 μm. fAβ₄₂ and Iba1were stained green and red, respectively. Nuclei were stained blue with DAPI. Merged images of all three channels were shown in a4 and b4. Merged image (b4) indicated Aβ₄₂ proteins were present within the cytoplasm of iMG cells. (B) The Aβ₄₂ protein uptake was quantified by flow cytometry. Representative image of three experiments were shown, where the blue graph was for control and red for iMG cells with engulfed fAβ₄₂. (C) Percentage of cells with engulfed fAβ₄₂ as measured by flow cytometry. The result was from three independent experiments, and the error bars represented ± SD. *p ≤ 0.05 was considered as significant.