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. 2021 Jan 28;11(3):jkab022. doi: 10.1093/g3journal/jkab022

Figure 4.

Figure 4

Heterochronic phenotypes associated with RNAi of C. sulstoni lin-14, lin-28, hbl-1, lin-46, and lin-29. (A) Panels from left to right: representative DIC images of C. sulstoni L4 hypodermis of animals fed control (empty vector), lin-14, lin-28, and hbl-1 RNAi, respectively. (B) Quantification of observed L4 alae phenotypes in (A). Scale bars = 10 µM. (C) Panels from left to right: representative DIC images of C. sulstoni adult hypodermis of animals fed control (empty vector), lin-46, and lin-29 RNAi, respectively. Quantification of complete adult alae (D) and adult alae branching (E) phenotypes observed in (C). Scale bars = 10 µM. Note: the control (empty vector) and lin-46 RNAi animals used for (D) were also used for (E).