Table 2.
Plasmids used in this study
| Laboratory strain number | Plasmid name | Relevant markers | Source |
|---|---|---|---|
| AG17 | pMSH2 | MSH2-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| pRS413 | HIS3 CEN6 ARSH4 ampr | Sikorski and Hieter (1989) | |
| AG122 | pGAL-MSH2 | PGAL10-MSH2-HA 2µ HIS3 ampr | Gammie et al. (2007) |
| pRS423 | 2µ HIS3 ampr | Sikorski and Hieter (1989) | |
| AG86 | pMSH2-D524Y | msh2-D524Y-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG375 | pMSH2-E194G | msh2-E194G-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG207 | pMSH2-C195R | msh2-C195R-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG495 | pMSH2-C195Y | msh2-C195Y-HA HIS3 CEN6 ARSH4 ampr | Arlow et al. (2013) |
| AG486 | pMSH2-L183P | msh2-L183P-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG507 | pMSH2-C345F | msh2-C345F-HA HIS3 CEN6 ARSH4 ampr | Arlow et al. (2013) |
| AG208 | pMSH2-C345R | msh2-C345R-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG209 | pMSH2-C345Y | msh2-C345Y-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG418 | pMSH2-G350R | msh2-G350R-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG417 | pMSH2-T347I | msh2-T347I-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG421 | pMSH2-A618V | msh2-A618V-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG497 | pMSH2-D621G | msh2-D621G-HA HIS3 CEN6 ARSH4 ampr | Arlow et al. (2013) |
| AG422 | pMSH2-D621N | msh2-D621N-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG419 | pMSH2-R371S | msh2-R371S-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG461 | pMSH2-G711D | msh2-G711D-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG239 | pMSH2-G711R | msh2-G711R-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG31 | pMSH2-P640L | msh2-P640L-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG487 | pMSH2-P640T | msh2-P640T-HA HIS3 CEN6 ARSH4 ampr | Arlow et al. (2013) |
| AG93 | pMSH2-C716F | msh2-C716F-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG211 | pMSH2-C716R | msh2-C716R-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG420 | pMSH2-L521P | msh2-L521P-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG496 | pMSH2-R542L | msh2-R542L-HA HIS3 CEN6 ARSH4 ampr | Arlow et al. (2013) |
| AG29 | pMSH2-R542P | msh2-R542P-HA HIS3 CEN6 ARSH4 ampr | Gammie et al. (2007) |
| AG124 | pGBD-MSH2 | GBD-MSH2-HA TRP1 2µ ampr | Gammie et al. (2007) |
| pGBD-C2 | GBD TRP1 2µ ampr | James et al. (1996) | |
| AG113 | pGAD-MSH6 | GAD-MSH6 LEU2 2µ ampr | Gammie et al. (2007) |
| AG378 | pGBD-MSH2-E194G | GBD-msh2-E194G-HA TRP1 2µ ampr | Gammie et al. (2007) |
| AG234 | pGBD-MSH2-C195R | GBD-msh2-C195R-HA TRP1 2µ ampr | Gammie et al. (2007) |
| AG504 | pGBD-MSH2-C195Y | GBD-msh2-C195Y-HA TRP1 2µ ampr | Arlow et al. (2013) |
| AG491 | pGBD-MSH2-L183P | GBD-msh2-L183P-HA TRP1 2µ ampr | Arlow et al. (2013) |
| AG518 | pGBD-MSH2-C345F | GBD-msh2-C345F-HA TRP1 2µ ampr | Arlow et al. (2013) |
| AG235 | pGBD-MSH2-C345R | GBD-msh2-C345R-HA TRP1 2µ ampr | Gammie et al. (2007) |
| AG236 | pGBD-MSH2-C345Y | GBD-msh2-C345Y-HA TRP1 2µ ampr | Gammie et al. (2007) |
| AG433 | pGBD-MSH2-G350R | GBD-msh2-G350R-HA TRP1 2µ ampr | Gammie et al. (2007) |
| AG432 | pGBD-MSH2-T347I | GBD-msh2-T347I-HA TRP1 2µ ampr | Gammie et al. (2007) |
| AG448 | pGBD-MSH2-A618V | GBD-msh2-A618V-HA TRP1 2µ ampr | Gammie et al. (2007) |
| AG501 | pGBD-MSH2-D621G | GBD-msh2-D621G-HA TRP1 2µ ampr | Arlow et al. (2013) |
| AG449 | pGBD-MSH2-D621N | GBD-msh2-D621N-HA TRP1 2µ ampr | Gammie et al. (2007) |
| AG446 | pGBD-MSH2-R371S | GBD-msh2-R371S-HA TRP1 2µ ampr | Gammie et al. (2007) |
| AG456 | pGBD-MSH2-G711D | GBD-msh2-G711D-HA TRP1 2µ ampr | Gammie et al. (2007) |
| AG265 | pGBD-MSH2-G711R | GBD-msh2-G711R-HA TRP1 2µ ampr | Gammie et al. (2007) |
| AG135 | pGBD-MSH2-P640L | GBD-msh2-P640L-HA TRP1 2µ ampr | Gammie et al. (2007) |
| AG492 | pGBD-MSH2-P640T | GBD-msh2-P640T-HA TRP1 2µ ampr | Arlow et al. (2013) |
| AG141 | pGBD-MSH2-C716F | GBD-msh2-C716F-HA TRP1 2µ ampr | Gammie et al. (2007) |
| AG215 | pGBD-MSH2-C716R | GBD-msh2-C716R-HA TRP1 2µ ampr | Gammie et al. (2007) |
| AG447 | pGBD-MSH2-L521P | GBD-msh2-L521P-HA TRP1 2µ ampr | Gammie et al. (2007) |
| AG130 | pGBD-MSH2-D524Y | GBD-msh2-D524Y-HA TRP1 2µ ampr | Gammie et al. (2007) |
| AG500 | pGBD-MSH2-R542L | GBD-msh2-R542L-HA TRP1 2µ ampr | Arlow et al. (2013) |
| AG132 | pGBD-MSH2-R542P | GBD-msh2-R542P-HA TRP1 2µ ampr | Gammie et al. (2007) |
| MR5893 | pMSH2-K65A | MSH2-K65A-HA HIS3 CEN6 ARSH4 ampr | Gammie Laboratory |
| MR5904 | pMSH2-K75A | MSH2-K75A-HA HIS3 CEN6 ARSH4 ampr | Gammie Laboratory |
| MR5894 | pMSH2-K96A | MSH2-K96A-HA HIS3 CEN6 ARSH4 ampr | Gammie Laboratory |
| MR5905 | pMSH2-K193A | MSH2-K193A-HA HIS3 CEN6 ARSH4 ampr | Gammie Laboratory |
| MR5895 | pMSH2-K404A | MSH2-K404A-HA HIS3 CEN6 ARSH4 ampr | Gammie Laboratory |
| MR5896 | pMSH2-K405A | MSH2-K405A-HA HIS3 CEN6 ARSH4 ampr | Gammie Laboratory |
| MR5897 | pMSH2-K549A | MSH2-K549A-HA HIS3 CEN6 ARSH4 ampr | Gammie Laboratory |
| MR5898 | pMSH2-K555A | MSH2-K555A-HA HIS3 CEN6 ARSH4 ampr | Gammie Laboratory |
| MR5899 | pMSH2-K564K | msh2-K564K-HA HIS3 CEN6 ARSH4 ampr | Gammie Laboratory |
| MR5900 | pMSH2-K873A | MSH2-K873A-HA HIS3 CEN6 ARSH4 ampr | Gammie Laboratory |
| MR5901 | pMSH2-K875A | MSH2-K875A-HA HIS3 CEN6 ARSH4 ampr | Gammie Laboratory |
| AG569 | pGAD-SAN1-C279S | GAD- san1-C279S LEU2 2µ ampr | (Arlow et al. 2013) |
| AG342 | pGAL-MSH2-R542P | PGAL10-msh2-R542P-HA 2µ HIS3 ampr | (Gammie et al. 2007) |
| pRS415 | LEU2 CEN6 ARSH4 ampr | (Sikorski and Hieter 1989) | |
| MR5919 | pMSH6-MYC | MSH6-myc CEN6 ARSH4 LEU2 ampr | Gammie Laboratory |