Table 3.
Missense substitutions causing increased turnover of Msh2 and their ability to interact with Msh6
| Cluster location (domains)a | Yeast position | Mismatch repairb | Interaction with Msh6b | Levels lowered in absence of Msh6 |
|---|---|---|---|---|
| Wild-type | + | ++ | ++ | |
| Connector/levers (2/3) | E194G | ++/– | ++ | ++ |
| C195R | – | – | – | |
| C195Y | – | – | – | |
| L183P | – | – | – | |
| C345F | – | – | – | |
| C345R | – | – | – | |
| C345Y | – | – | – | |
| Levers/ATPase (3/5) | G350R | – | – | – |
| T347I | ++/– | ++ | ++ | |
| A618V | – | + | ++ | |
| D621G | – | – | – | |
| D621N | – | – | – | |
| R371S | – | – | – | |
| G711D | – | – | – | |
| G711R | – | – | – | |
| Levers/ATPase (3/5) | P640L | – | – | – |
| P640T | – | – | – | |
| C716F | – | – | – | |
| C716R | – | – | – | |
| Clamp/levers (3/4) | L521P | – | ++ | ++ |
| D524Y | – | + | ++ | |
| R542L | – | ++ | ++ | |
| R542P | – | ++ | ++ |
a
Domains correspond to the human MutSα structure (Warren et al. 2007).
b
The mismatch repair defects and interactions with Msh6 for most of the variants were first described in our previous publications (Gammie et al. 2007; Arlow et al. 2013).