Figure S4.

Expression of NEO1, NET1, RGMA and RGMB, related to Figure 3

(A) Protein expression of NET1, NEO1 and RGMB in the adult mouse brain detected by western blot analysis. (B) Sagittal overview of the adult mouse brain. (C)In situ hybridization for NEO1, NET1, RGMA and RGMB in sagittal sections from the adult mouse brain (obtained from the Allen Brain Atlas (brain-map.org)). Regions of interest are indicated in boxed regions in B: (i) anterodorsal nucleus of the thalamus, (ii) cerebellum and (iii) olfactory nucleus. Images are obtained from the Allen Brain Atlas. Olf bulb, olfactory bulb; ctx ant, anterior half of the cortex; ctx post, posterior half of the cortex; hip, hippocampus; Th, thalamus; AON, anterior olfactory nucleus; ACB, nucleus accumbens. Scale bar = 500 μm. (D) scRNAseq dataset analysis (Mizrak et al 2019) for co-expression of RGMA/B, NEO1 and NET1 in adult V-SVZ. Single-cell expression levels of cluster-specific marker genes in adult ventricular-subventricular zone (V-SVZ) cells plotted on UMAP embedding (Cldn10, Mog, Ccnd2, Tmem119, Meg3, Egfl7, Ccdc153, Vtn, Pdgfra, Fyn). In addition, expression levels of Neogenin (NEO1), Netrin-1 (NET1), RGMA and RGMB are shown. Clusters marked as [clusterID]. OPC, oligodendrocyte precursor; COP, committed oligodendrocyte precursors.