FIGURE 4.
CCL2 enhances induction of Arabidopsis defense gene expression in response to B. cinerea. Four-week-old Arabidopsis plants (WT, CCL2, and CCL2-Y92A lines) were spray-inoculated with B. cinerea (5 × 105 spores mL–1). Leaves were harvested at 0 and 1 dpi for RNA extraction. Transcript levels of OBP2 (A), PDF1.2 (B), and PR-1 (C) were determined by qPCR. Data were normalized with regard to the Arabidopsis reference gene expG. Data represent mean values ± SE of three independent experiments. The letters a and b signify a between-group difference at the P ≤ 0.05 level. Two-way ANOVA and post hoc analysis by Tukey’s multiple-comparison test were used to calculate significant differences between transgenic lines and WT plants.