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. 2021 Apr 9;9:583492. doi: 10.3389/fcell.2021.583492

FIGURE 3.

FIGURE 3

MKL1 regulates a pro-hypertrophic cue from macrophages. (A–D) RAW cells were transfected with siRNA targeting MKL1 or scrambled siRNA (SCR). Conditioned media were collected 48 h after transfection and applied to primary neonatal rat ventricular myocytes along with endothelin. Knockdown efficiencies were verified by qPCR and Western (A). ANP (B), BNP (C), and β-MHC (D) levels were examined by qPCR. (E–G) RAW cells were treated with or without CCG-1423 (10 μM) for 24 h. Conditioned media were collected 48 h after transfection and applied to primary neonatal rat ventricular myocytes along with endothelin. ANP (E), BNP (F), and β-MHC (G) levels were examined by qPCR. (H–J) Conditioned media were collected from WT and MFCKO bone marrow derived macrophages and applied to primary neonatal rat ventricular myocytes along with endothelin. ANP (E), BNP (F), and β-MHC (G) levels were examined by qPCR. Data represent mean ± SD. *p < 0.05, One-way ANOVA with post hoc Scheffe test.