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Reaction time for RT-RPA-VF assays targeting the CDV N gene
| Time | No. positive/ no. replications | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Synthesized RNA transcript dilution (5 × copies/μl) | |||||||||
| 107 | 106 | 105 | 104 | 103 | 102 | 101 | 100 | N | |
| 15 min | 3/3 | 3/3 | 3/3 | 3/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| 20 min | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 0/3 | 0/3 | 0/3 |
| 25 min | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 1/3 | 0/3 | 0/3 |
| 30 min | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 0/3 | 0/3 |
| 35 min | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 0/3 | 0/3 |
