Figure 4.

SAT mitochondria-related proteins were upregulated following surgical intervention. Protein enrichment for biological pathways using differentially expressed proteins (nominal P < 0.1) associated with the weight-loss percentage following surgery-induced weight loss in KOBS (n = 29) (A), the top 10 significant findings from the ingenuity pathway analysis (IPA) tool (P < 0.001) are shown. For several pathways, IPA provided z-scores for pathway directionality by calculating the observed number of “activated” genes (z-score > 0), “inhibited” genes (z-score < 0), or no directionality prediction (z-score = 0). Z-scores > 2 or < −2 were considered statistically significant. Results ranked according to statistical significance. In (B), a heat map showing the differential expression of the individual proteins in the oxidative phosphorylation (OXPHOS) pathway associated with weight-loss percentage following surgery-induced weight loss. Log₂-fold changes indicated for the 5 primary complexes of the electron transport chain proteins. Only measured proteins are shown. The color in the heat maps reflect the differential expressions associated with the weight-loss percentage before and after weight loss, where red indicates up- and blue indicates downregulation. Asterisks indicate statistically significant differential expressions: **P < 0.05; *P < 0.1. Abbreviations: c, cytochrome c; q, ubiquinone.