PCAT6 is upregulated in liver cancer tissues and associated with clinicopathological characteristics of patients with liver cancer. (A) Expression of PCAT6 in liver cancer tissues (n=369) and normal tissues (n=160) from the GEPIA database. (B) Association between PCAT6 expression and overall survival rate of patients with liver cancer was analyzed using GEPIA database. (C) RT-qPCR was performed to examine the expression of PCAT6 in HCCT (n=117) and matched adjacent normal tissues. (D) RT-qPCR was used to detect the expression of PCAT6 in liver cancer cell lines (MHCC97H, HepG2 and Huh7) and normal liver epithelial cells THLE-3. *P<0.05 and ***P<0.001 compared with the normal tissues; ##P<0.01 and ###P<0.001 compared with the THLE-3 cell line. PCAT6, prostate cancer-associated transcript 6; GEPIA, Gene Expression Profiling Interactive Analysis; RT-qPCR, reverse transcription-quantitative PCR; HCCT, liver cancer tissue; ANT, adjacent normal tissues; LIHC, liver hepatocellular carcinoma; TPM, transcripts per million; HR, hazard ratio.