Figure 2.

Knockdown of PCAT6 inhibits cell proliferation and invasion in liver cancer in vitro. (A) Both HepG2 and Huh7 cells were transfected with sh-PCAT6 or sh-NC and reverse transcription-quantitative PCR was used to detect the expression of PCAT6 in both cells. (B and C) Cell Counting Kit-8 assay was used to examine the cell proliferation in both HepG2 and Huh7 cell transfected with sh-PCAT6. (D) After transfection, colony formation of HepG2 and Huh7 was determined. (E) Transwell assay was applied to detect the invasive activity of liver cancer cells after transfection with sh-PCAT6 and sh-NC. *P<0.05, **P<0.01 and ***P<0.001 compared with sh-NC. PCAT6, prostate cancer-associated transcript 6; sh-, short hairpin-; NC, negative control.