Figure 4.

Inhibition of miR-326 reverses the inhibitory effect of PCAT6-knockdown on liver cancer cell proliferation and invasion ability of both HepG2 and Huh7 cells. (A) Reverse transcription-quantitative PCR was used to detect the expression of miR-326 in both HepG2 and Huh7 cells. (B and C) Cell Counting Kit-8 assay was used to measure the cell proliferation in both HepG2 and Huh7 cells after transfection with miR-Inh and sh-PCAT6. (D) After transfection, HepG2 and Huh7 cell colony formation abilities were determined. (E) Transwell assays were applied to detect the invasive activity of HepG2 and Huh7 cells after transfection with miR-Inh and sh-PCAT6. *P<0.05, **P<0.01 and ***P<0.001 compared with control. miR-, microRNA; PCAT6, prostate cancer-associated transcript 6; miR-Inh, miR-326 inhibitor; sh-, short hairpin.