Figure 5.

PCAT6 contributes to liver cancer cell proliferation and invasion by regulating the miR-326/hnRNPA2B1 axis. (A) Western blotting was used to detect the protein level of hnRNPA2B1 in both liver cancer cell after transfection with hnRNPA2B1 shRNA (sh-hnRNPA2B1), combined hnRNPA2B1 shRNA and miR-326 inhibitor (sh-hnRNPA2B1+miR-Inh), combined hnRNPA2B1 shRNA and pcDNA3.1-PCAT6 (sh-hnRNPA2B1+PCAT6). (B and C) After transfection, the proliferation ability of both HepG2 and Huh7 cells were determined by using a Cell Counting Kit-8 assay and (D and E) colony formation assay. (F and G) Cell invasion was detected using a Transwell assay. *P<0.05, **P<0.01 and ***P<0.001. PCAT6, prostate cancer-associated transcript 6; miR-, microRNA; hnRNPA2B1, heterogeneous nuclear ribonucleoprotein A2B1; sh-, short hairpin-; miR-Inh, miR-326 inhibitor.