Skip to main content
. 2021 Apr 8;21(6):457. doi: 10.3892/ol.2021.12718

Figure 5.

Figure 5.

miR-133a-3p inhibits the proliferation, invasion and migration of non-small cell lung cancer cells by negatively regulating ERBB2. (A) At the mRNA level, the expression levels of miR-133a-3p were increased in the miR-133a-3p mimic and miR-133a-3p mimic + pcDNA-ERBB2 groups. ERBB2 expression was increased in the pcDNA-ERBB2 group, while it was decreased in cells transfected with miR-133a-3p mimic. (B) ERBB2 protein expression was suppressed in cells transected with miR-133a-3p mimic. (C) MTT assay data demonstrated that upregulated miR-133a-3p and ERBB2 in combination preserved the H1299 cell proliferation activity compared with cells transfected with pcDNA-ERBB2 alone. (D) The invasion ability was attenuated in H1299 cells transfected with miR-133a-3p mimic and pcDNA-ERBB2 in combination compared with cells transfected with pcDNA-ERBB2 alone. Magnification, ×400. (E) Wound healing assays suggested that co-transfection with miR-133a-3p mimic and pcDNA-ERBB2 decreased the wound closure ratio in H1299 cells compared with transfection with pcDNA-ERBB2 alone. Magnification, ×400. *P<0.05 vs. NC. ERBB2, erb-b2 receptor tyrosine kinase 2; miR-133a-3p, microRNA-133a-3p; NC, negative control.