KEY RESOURCES TABLE
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Rat polyclonal antibody against CD13 | Acris Antibodies | Cat#SM2298P |
Rabbit polyclonal antibody against phospho-myosin light chain (pMLC) | Cell Signaling Technologies | Cat#3671 |
Mouse polyclonal antibody against GAPDH | Sigma-Aldrich | Cat#G8795 |
CF568 conjugated Donkey Anti-Rat IgG (H+L) Antibody | Biotium | Cat#20092 |
Alexa fluorophore 488-conjugated Phalloidin | Thermo Fisher Scientific | Cat#A12379 |
Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) | Jackson Immuno Research Laboratories | Cat#111035144 |
Chemicals, Peptides and Recombinant Proteins | ||
Hoechst 33258 | Thermo Fisher Scientific | Cat#H3569 |
Tetramethylrhodamine-conjugated 2000 kDa Dextran | Thermo Fisher Scientific | Cat#D7139 |
Texas-Red conjugated 70 kDa Dextran | Thermo Fisher Scientific | Cat#D1830 |
Qtracker 655 vascular labels | Thermo Fisher Scientific | Cat#Q21021MP |
Carbomer 940 | Spectrum Chemical | Cat#C1477 |
Fasudil HCl (HA-1077) | Alfa Aesar | Cat#J60751 |
6-Carboxfluoreceine diacetate (CFDA) | Sigma-Aldrich | Cat#C5041 |
Experimental Models: Organisms/Strains | ||
Wildtype mice | Harlan laboratories | C57BL/6JHsd mice |
Lifeact-EGFP mice | Laboratory of Roland Wedlich-Soldner, University of Münster, Germany (Riedl et al., 2010) | NA |
Software and Algorithms | ||
MotionTracking | Morales-Navarrete et al., 2015 | http://motiontracking.mpi-cbg.de |
Imaris | BitPlane, Inc. | NA |
OpenFoam | NA | http://www.openfoam.org |
Fiji | Schindelin et al., 2012 | https://fiji.sc |
ANSYS ICEM CFD | Ansys, Inc. | NA |
FitModel | Zeigerer et al., 2012 | http://pluk.mpi-cbg.de/projects/fitmodel |
Morpheus | Starruss et al., 2014 | https://gitlab.com/morpheus.lab/morpheus |
Matlab | The MathWorks, Inc. | NA |
GraphPad 7 | GraphPad Software, Inc. | NA |