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. 2021 Apr 28;6:37. Originally published 2021 Feb 22. [Version 2] doi: 10.12688/wellcomeopenres.16619.2

Table 2. Expected results logged in the BoneJ result table comparing BoneJ2 and BoneJ1 output.

Due to the stochastic nature of sampling both degree of anisotropy (DA) and ellipsoid factor (EF) vary between runs in an image- and setting-specific manner. Modern Anisotropy and Ellipsoid Factor 16 plugins differ in implementation from those in BoneJ1, so some minor variation in output is expected. Users are strongly encouraged to run sensitivity analyses. to select settings for Anisotropy and Ellipsoid Factor (as we have done) 16 that produce a stable result on their images. Note that Thickness, Volume Fraction and Connectivity are direct ports from BoneJ1 to BoneJ2 and have no stochastic character, so their results are expected to be invariant for the same input image. BoneJ2’s Purify uses Particle Analyser’s improved ConnectedComponents code 19 and completes in ~ 1 s compared to ~ 15 s in BoneJ1.

Image BoneJ2
( styloid-r11)
BoneJ 1.4.3
DA 0.51675 0.56665
BV (mm 3) 25.92792 25.92792
TV (mm 3) 62.21983 62.21983
BV/TV 0.41671 0.41671
Tb.Th Mean (mm) 0.21246 0.21246
Tb.Th Std. Dev. (mm) 0.06347 0.06347
Tb.Th Max (mm) 0.43277 0.43277
Tb.Sp Mean (mm) 0.47014 0.47014
Tb.Sp Std. Dev. (mm) 0.14888 0.14888
Tb.Sp Max (mm) 0.96242 0.96242
Median EF -0.14632 NaN (mean, -0.21451)
Max EF 0.91779 0.91695
Min EF -0.84867 -0.93064
Euler char (χ) -291 -291
Corr. Euler (χ – Δχ) -238.375 -238.375
Connectivity 239.375 239.375
Conn.D (mm 3) 3.84725 3.84725