Figure 1.

Rapid HSPG-mediated tumor killing by AU-011. 92.1 cells were preplated at 2.5×10⁴/500 μL in a 24-well plate and allowed to grow for 48 hours. A total of 7.5 μg/mL (300 pmol/L) of AU-011 was incubated for 1 hour ± 1 mg/mL heparin at 4°C and then added to the cells for 1 hour at 37°C. IR700 dye was also added in an amount equivalent to that which is conjugated to 300 pmol/L of AU-011. After 1 hour, cells were washed twice and then either kept in the dark or exposed to 25 J/cm² of 690 nm light. Images were acquired within 15 minutes of light treatment.