Figure 2.

HSPG specificity of AU-011 activity. HeLa (A) or 92.1MEL (B) cells were combined at a 1:1 ratio with either CHO-K1 (gray bar) or pgsA-745 cells (white bar) and coincubated with AU-011 at 300 pmol/L for 1 hour at 4°C. Cells were washed and then exposed to 25 J/cm² or kept in the dark (0 J/cm²). Prior to combining cell lines, human cell lines were incubated with CFSE, and hamster cell lines were incubated with CellTrace Violet to distinguish the populations for flow cytometry analysis (example of the analysis provided in Supplementary Fig. S5). Data are reported as % dead as determined by viability staining. Values plotted are the mean of two experiments performed in triplicate and error bars represent the SEM.