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. 2021 Apr 9;9:655866. doi: 10.3389/fcell.2021.655866

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Copyright © 2021 Vitorino Carvalho, Soler, Thélie, Grasseau, Cordeiro, Tomas, Teixeira-Gomes, Labas and Blesblois.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Principal component analysis (PCA) of protein abundances obtained by GeLC-MS/MS with unique gene identification combined with fertility status and semen mass motility. Only DAP with a unique gene and an annotation (exclusion of LOC proteins) were included in this analysis. One significant DAP per cluster was retained and the mean signal calculated based on the three technical replicates obtained for each animal. Animals are revealed by their identification number and their fertility status (F = fertile; SF = subfertile). Animals are represented in gray and protein contribution as well as semen parameters in blue.