TABLE 1.
Differentially abundant proteins between fertile (F) and subfertile (SF) meat-line chickens identified by GeLC-MS/MS.
|
Spectral counting |
API |
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| # | Gene description | Accession number | Molecular weight (kDa) | Gene symbol | p-value | F/SF FC | p-value | F/SF FC |
| 1.51 | Tubulin beta-1 chain isoform X2 [Electrophorus electricus] | XP_026866564.1 | 49 | TUBB1 | 0.240 | 0.80 | 0.029 | 0.70 |
| 1.53 | Beta tubulin, partial [Gillichthys mirabilis] | AAL24510.1 | 17 | 0.087 | 0.70 | 0.011 | 1.90 | |
| 2.35 | Tubulin alpha-2 chain isoform X2 [Numida meleagris] | XP_021246036.1 | 43 | LOC110395670 | 0.380 | 1.30 | 0.039 | 0.60 |
| 2.38 | Tubulin alpha-8 chain-like [Cebus capucinus imitator] | XP_017390722.1 | 53 | LOC108309094 | 0.210 | 0.80 | 0.043 | 0.70 |
| 2.53 | Tubulin alpha-8 chain-like [Sphaeramia orbicularis] | XP_029994229.1 | 51 | LOC115422205 | 0.030 | 0.50 | 0.005 | 0.40 |
| 6.4 | Hexokinase-3 isoform X1 [Coturnix japonica] | XP_015731713.1 | 109 | HK3 | 0.700 | 1.10 | 0.002 | 1.90 |
| 6.6 | Hexokinase-3-like [Tyto alba alba] | XP_032839590.1 | 105 | LOC104357108 | 0.091 | 6.80 | 0.049 | 2.60 |
| 7.25 | Hypothetical protein A6R68_14483, partial [Neotoma lepida] | OBS74971.1 | 39 | 0.009 | 2.80 | 0.016 | 3.30 | |
| 8.1 | Fructose-bisphosphate aldolase A isoform X2 [Equus caballus] | XP_003362760.1 | 39 | ALDOA | 0.065 | 1.10 | 0.010 | 1.60 |
| 8.1 | Fructose-bisphosphate aldolase C, partial [Lepidosiren paradoxa] | BAD17883.1 | 36 | 0.019 | 2.80 | 0.170 | 1.40 | |
| 8.14 | Fructose-bisphosphate aldolase A-like, partial [Apteryx rowi] | XP_025921567.1 | 29 | LOC112966083 | 0.019 | 1.80 | 0.100 | 1.20 |
| 8.2 | Fructose-bisphosphate aldolase A [Corvus moneduloides] | XP_031953954.1 | 39 | ALDOA | 0.027 | 1.30 | 0.004 | 1.50 |
| 8.3 | Fructose-bisphosphate aldolase A-like [Meleagris gallopavo] | XP_019467335.1 | 8 | LOC104917114 | 0.096 | 1.20 | 0.003 | 1.70 |
| 8.4 | Fructose-bisphosphate aldolase A isoform X1 [Desmodus rotundus] | XP_024416190.1 | 45 | ALDOA | 0.200 | 1.10 | 0.003 | 1.80 |
| 8.5 | Fructose-bisphosphate aldolase A-like [Numida meleagris] | XP_021239723.1 | 14 | LOC110392092 | 0.140 | 1.10 | 0.043 | 1.50 |
| 8.6 | Aldolase, fructose-bisphosphate A S homeolog [Xenopus laevis] | NP_001079649.1 | 39 | ALDOA | 0.340 | 1.10 | 0.006 | 1.70 |
| 8.7 | Fructose-bisphosphate aldolase A [Pogona vitticeps] | XP_020654424.1 | 40 | ALDOA | 0.150 | 1.20 | 0.042 | 1.50 |
| 8.8 | Fructose-bisphosphate aldolase A, partial [Pygoscelis adeliae] | KFW62523.1 | 17 | LOC103925596 | 0.046 | 1.40 | 0.150 | 1.20 |
| 8.9 | Fructose-bisphosphate aldolase C [Coturnix japonica] | XP_015736436.1 | 39 | ALDOC | 0.024 | 1.50 | 0.017 | 1.50 |
| 10.1 | Creatine kinase B-type [Gallus gallus] | NP_990641.1 | 43 | CKB | 0.011 | 1.50 | 0.046 | 1.50 |
| 10.11 | Creatine kinase B-type isoform X1 [Nanorana parkeri] | XP_018412106.1 | 43 | CKB | 0.420 | 1.30 | 0.044 | 1.60 |
| 10.13 | Creatine kinase M-type [Papio anubis] | XP_003915756.1 | 43 | 0.094 | 2.00 | 0.003 | 6.20 | |
| 10.2 | Creatine kinase B-type isoform X1 [Gallus gallus] | XP_015142790.1 | 42 | CKB | 0.043 | 1.40 | 0.055 | 1.50 |
| 10.3 | Creatine kinase B-type isoform X1 [Apteryx mantelli mantelli] | XP_013796252.1 | 43 | CKB | 0.013 | 1.40 | 0.160 | 1.30 |
| 10.5 | Creatine kinase, brain, isoform CRA_a [Homo sapiens] | EAW81820.1 | 43 | CKB | 0.020 | 1.40 | 0.790 | 0.90 |
| 10.6 | Creatine kinase B-type isoform X1 [Chaetura pelagica] | XP_010006458.1 | 43 | CKB | 0.011 | 1.70 | 0.150 | 1.50 |
| 10.9 | Creatine kinase B-type [Mesocricetus auratus] | XP_005068378.1 | 43 | CKB | 0.048 | 1.40 | 0.330 | 0.70 |
| 13.3 | Hypothetical protein CIB84_011751 [Bambusicola thoracicus] | POI24503.1 | 12 | 0.160 | 1.20 | 0.010 | 1.70 | |
| 14.1 | Ovotransferrin [Gallus gallus] | P02789.2 | 78 | TF | 0.028 | 1.40 | 0.260 | 1.40 |
| 14.2 | Ovotransferrin BC type [Gallus gallus] | BAE16339.1 | 78 | TF | 0.042 | 1.40 | 0.390 | 1.30 |
| 18.1 | ATP synthase subunit alpha, mitochondrial, partial [Colius striatus] | XP_010196224.1 | 58 | ATP5F1A | 0.001 | 0.70 | 0.760 | 1.00 |
| 18.5 | ATP synthase subunit alpha, mitochondrial-like [Arvicanthis niloticus] | XP_034353318.1 | 60 | LOC117705093 | 0.005 | 0.60 | 0.008 | 1.60 |
| 22.1 | Keratin 1 [Homo sapiens] | AAG41947.1 | 66 | KRT1 | 0.019 | 1.60 | 0.095 | 1.80 |
| 22.3 | Keratin, type II cytoskeletal 1 isoform X2 [Macaca mulatta] | XP_001098182.2 | 65 | KRT1 | 0.034 | 2.10 | 0.360 | 1.40 |
| 22.8 | Keratin, type II cytoskeletal 6A isoform X2 [Pongo abelii] | XP_024113011.1 | 57 | LOC100449906 | 0.002 | 4.30 | 0.012 | 2.70 |
| 24.1 | Heat shock protein Hsp70 [Gallus gallus] | AAN18280.1 | 70 | HSPA2 | 0.045 | 1.60 | 0.750 | 1.10 |
| 24.7 | Heat shock 70 kDa protein [Gallus gallus] | BBO36590.1 | 70 | HSPA2 | 0.024 | 2.00 | 0.910 | 1.00 |
| 27.2 | Hypothetical protein N333_09691, partial [Nestor notabilis] | KFQ42654.1 | 45 | GOT2 | 0.530 | 0.90 | 0.005 | 1.70 |
| 32.1 | Hypothetical protein ASZ78_016751 [Callipepla squamata] | OXB67628.1 | 45 | 0.003 | 1.80 | 0.140 | 1.30 | |
| 32.11 | Phosphoglycerate kinase 1 [Paralichthys olivaceus] | XP_019941634.1 | 44 | PGK1 | 0.110 | 1.80 | 0.040 | 1.80 |
| 32.2 | Phosphoglycerate kinase 1 [Ceratotherium simum simum] | XP_004442122.1 | 45 | LOC101387600 | 0.001 | 1.60 | 0.010 | 1.80 |
| 32.3 | Phosphoglycerate kinase 1 [Echeneis naucrates] | XP_029374590.1 | 44 | PGK1 | 0.020 | 1.60 | 0.045 | 1.50 |
| 32.8 | Phosphoglycerate kinase 2-like [Ictidomys tridecemlineatus] | XP_005318639.1 | 45 | LOC101964802 | 0.002 | 1.60 | 0.014 | 1.70 |
| 33.1 | Keratin 10 [Homo sapiens] | AAH34697.1 | 59 | KRT10 | 0.010 | 1.50 | 0.900 | 1.00 |
| 33.2 | Keratin, type I cytoskeletal 9 [Homo sapiens] | NP_000217.2 | 62 | KRT9 | 0.022 | 2.30 | 0.170 | 2.00 |
| 33.3 | Keratin 14 [Homo sapiens] | AAH02690.1 | 52 | KRT14 | 0.000 | 4.20 | 0.180 | 1.70 |
| 46 | Unnamed protein product, partial [Gallus gallus] | CAA30161.1 | 54 | LOC107051274 | 0.000 | 0.30 | 0.400 | 0.80 |
| 49.1 | Mitochondria-eating protein isoform X1 [Gallus gallus] | XP_004936087.1 | 62 | SPATA18 | 0.001 | 1.60 | 0.094 | 1.40 |
| 56.1 | Citrate synthase, mitochondrial isoform X2 [Gallus gallus] | XP_015155774.1 | 56 | CS | 0.020 | 1.40 | 0.003 | 1.90 |
| 59 | Hypothetical protein CIB84_000737 [Bambusicola thoracicus] | POI35507.1 | 30 | 0.013 | 1.60 | 0.420 | 1.20 | |
| 60.1 | Cytochrome c [Gallus gallus] | NP_001072946.1 | 12 | CYCS | 0.037 | 0.60 | 0.310 | 1.20 |
| 63.1 | Spermatid-specific manchette-related protein 1 isoform X2 [Gallus gallus] | XP_004937159.1 | 30 | CZH9orf24 | 0.030 | 0.30 | 0.180 | 0.60 |
| 65.1 | Complex I assembly factor ACAD9, mitochondrial [Gallus gallus] | NP_001006136.1 | 68 | ACAD9 | 0.009 | 1.60 | 0.980 | 1.00 |
| 65.2 | Acyl-CoA dehydrogenase family member 9, mitochondrial [Rhincodon typus] | XP_020383709.1 | 70 | ACAD9 | 0.015 | 3.00 | 0.032 | 4.60 |
| 68.4 | Hypothetical protein parPi_0021429 [Paroedura picta] | GCF58429.1 | 47 | 0.580 | 0.90 | 0.047 | 1.50 | |
| 69.3 | Long-chain specific acyl-CoA dehydrogenase, mitochondrial [Monopterus albus] | XP_020470903.1 | 50 | ACADL | 0.430 | 1.20 | 0.039 | 1.40 |
| 82.3 | Ornithine aminotransferase, mitochondrial [Phaethon lepturus] | XP_010295191.1 | 48 | OAT | 0.013 | 2.20 | 0.038 | 1.70 |
| 89.1 | T-complex protein 1 subunit zeta [Gallus gallus] | NP_001006216.1 | 58 | CCT6A | 0.014 | 1.50 | 0.540 | 1.20 |
| 89.4 | T-complex protein 1 subunit zeta [Phascolarctos cinereus] | XP_020862612.1 | 58 | CCT6A | 0.070 | 2.20 | 0.004 | 3.60 |
| 91.2 | Superoxide dismutase [Mn], mitochondrial, partial [Thamnophis sirtalis] | XP_013926038.1 | 18 | SOD2 | 0.072 | 0.80 | 0.039 | 0.70 |
| 92.1 | T-complex protein 1 subunit alpha, partial [Eudyptula novaehollandiae] | KAF1505402.1 | 62 | 0.710 | 1.10 | 0.044 | 1.60 | |
| 92.2 | Thermosome subunit beta [Turdus rufiventris] | KAF4796296.1 | 48 | 0.220 | 1.40 | 0.032 | 1.50 | |
| 92.3 | PREDICTED: T-complex protein 1 subunit alpha [Anolis carolinensis] | XP_003215812.1 | 60 | TCP1 | 0.560 | 1.20 | 0.033 | 1.60 |
| 93.1 | Hypothetical protein RCJMB04_35g11 [Gallus gallus] | CAG32769.1 | 55 | LOC107056139 | 0.130 | 2.00 | 0.022 | 2.40 |
| 98.1 | Tektin-3 [Gallus gallus] | XP_004946137.1 | 56 | TEKT3 | 0.067 | 0.00 | 0.031 | 0.00 |
| 103.1 | Peroxisomal carnitine O-octanoyltransferase [Gallus gallus] | XP_003640711.1 | 70 | CROT | 0.400 | 1.30 | 0.019 | 2.50 |
| 135.1 | T-complex protein 1 subunit delta [Gallus gallus] | NP_996761.1 | 58 | CCT4 | 0.590 | 1.20 | 0.035 | 4.00 |
| 135.2 | T-complex protein 1 subunit delta-like [Trachypithecus francoisi] | XP_033033221.1 | 58 | LOC117063540 | 0.100 | 2.00 | 0.002 | 3.10 |
| 138.1 | Tektin-4 [Gallus gallus] | XP_414831.1 | 53 | TEKT4 | 0.051 | 0.20 | 0.012 | 0.20 |
| 139 | Dynein light chain LC6, flagellar outer arm [Gallus gallus] | XP_001233550.2 | 10 | DYL2 | 0.180 | 1.60 | 0.008 | 3.50 |
| 148.1 | Lysozyme [Gallus gallus] | P00698.1 | 16 | LYZ | 0.001 | 0.30 | 0.001 | 0.40 |
| 153 | Apolipoprotein A-I [Gallus gallus] | AAA48597.1 | 31 | APOA1 | 0.008 | 0.50 | 0.510 | 0.90 |
| 178.1 | cAMP and cAMP-inhibited cGMP 3’,5’-cyclic phosphodiesterase 10A isoform X1 [Gallus gallus] | XP_004935608.1 | 116 | PDE10A | 0.260 | 1.60 | 0.034 | 2.80 |
| 187 | Transthyretin isoform 2 [Gallus gallus] | NP_001268427.1 | 19 | TTR | 0.013 | 0.60 | 0.025 | 0.70 |
| 217.1 | Plasminogen [Gallus gallus] | XP_419618.2 | 91 | PLG | 0.006 | 0.00 | 0.015 | 0.00 |
| 224.1 | Hypothetical protein N338_03343, partial [Podiceps cristatus] | KFZ67843.1 | 43 | 0.007 | 2.90 | 0.001 | 2.70 | |
| 253.1 | Annexin A1 [Gallus gallus] | NP_996789.1 | 39 | ANXA1 | 0.031 | 0.03 | 0.030 | 0.00 |
| 263 | Hypothetical protein ASZ78_013233 [Callipepla squamata] | OXB59879.1 | 23 | 0.021 | 2.10 | 0.007 | 2.10 | |
| 264 | Immunoglobulin heavy chain variable region, partial [Gallus gallus] | BAA11105.1 | 11 | 0.002 | 0.40 | 0.000 | 0.30 | |
| 275.1 | Nucleoside diphosphate kinase homolog 5 [Gallus gallus] | NP_001244300.2 | 24 | NME5 | 0.092 | 0.20 | 0.048 | 0.20 |
| 284.1 | Acetyl-CoA acetyltransferase, cytosolic [Gallus gallus] | NP_001034376.2 | 41 | ACAT2 | 0.058 | 4.20 | 0.027 | 6.10 |
| 293 | Alpha-2-HS-glycoprotein [Gallus gallus] | XP_422764.1 | 37 | AHSG | 0.028 | 0.00 | 0.014 | 0.00 |
| 310.1 | Disintegrin and metalloproteinase domain-containing protein 32-like [Gallus gallus] | XP_024998701.1 | 82 | ADAM32 | 0.027 | 4.20 | 0.026 | 3.50 |
| 311.1 | Hypothetical protein CIB84_002892 [Bambusicola thoracicus] | POI33356.1 | 51 | 0.019 | 0.00 | 0.025 | 0.00 | |
Significant abundance differences were characterized between F and SF animals by applying t-test and retaining only accession numbers with p-values ≤ 0.05 associated with a fold-change ratio (FC) ≤ 0.71 or ≥ 1.4. Significant peptides are indicated with bold characters within columns corresponding to spectral counting and API (Average Precursor Intensity) quantitative methods. #: number of the considered cluster (before the point) and peptides (after the point).