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The effects of S1 binding to the actin–compound complex in the presence and absence of MgATP. Normalized fluorescence of 2 μM pyrene-labeled F-actin (PFA) (gray) with S1 (0.7 S1/actin) only (pink). A, calculated (using Equation 5, hatched bars) and experimental (green) fluorescence of PFA.compound.S1 complex. B, the effect of 0.5 mM MgATP (peach) on the PFA.compound.S1 complex (green). [Compound] was 100 μM except for Flu and Dan (50 μM) and Mit (10 μM).
