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. 2021 Mar 24;7(4):242. doi: 10.3390/jof7040242

Figure 2.

Figure 2

Identification and genotyping of P. insidiosum by multiplex PCR. The multiplex PCR amplifies the rDNA sequence from a gDNA sample extracted from water-isolated P. insidiosum-suspected colonies. The amplicon sizes are assessed by using the capillary electrophoresis-based QIAxcel advanced system (Qiagen) (see the methods). The positive controls include gDNA samples extracted from P. insidiosum strains Pi08 (Clade-I genotype; amplicons: 490- and 660-bp bands), Pi35 (Clade-II genotype; amplicon: 660-bp band), and Pi45 (Clade-III genotype; amplicon: 800-bp band). The PCR reaction with no gDNA template serves as the negative control [no template control (NTC)]. The multiplex PCR results of 7 randomly-selected P. insidiosum-suspected organisms (IDs: BKDZ02, RCB01, RM9-06, CCS09, RT02, KCB01, and RCB06) are shown in the Figure.