. 2021 Mar 24;7(4):242. doi: 10.3390/jof7040242

Table 2.

Twenty-seven strains of P. insidiosum successfully isolated from 5 provinces in Thailand. Sampling locations (with GPS coordinates), water sources, strain IDs, sequence homology analyses, and species-level identification and biotyping (based on DNA barcodes, multiplex PCR, and mass spectrometry) are summarized in the Table.

Province (P. insidiosum Isolates)	Sampling Locations		Water Source (Collection Site)	Strain ID	DNA Barcodes						Genotype (Clade)		MALDI-TOF MS
	Code	GPS Coordinate			rDNA		cox1		cox2		DNA Barcode	Multiplex PCR	Score	Proteotype
	Code	GPS Coordinate			Accession	% Identity	Accession	% Identity	Accession	% Identity	DNA Barcode	Multiplex PCR	Score	Proteotype
Bangkok (n = 7)	BK01	13.770966, 100.516366	Pond#1 (zoo)	BKDZ01	LC556017	99.9	LC547937	94.3	LC549516	100.0	III	III	-	-
	BK01	13.770966, 100.516366	Pond#2 (zoo)	BKDZ02	LC556018	98.0	LC547938	94.1	LC549517	100.0	III	III	2.200	B
	BK04	13.929843, 100.568340	Pond#3 (park)	RT02	LC556073	99.9	LC547929	99.7	LC549523	100.0	II	II	-	-
	BK09	13.686055, 100.662055	Pond#4 (park)	RM9-02	LC556063	99.5	LC547930	100.0	LC549519	100.0	II	II	-	-
				RM9-03	LC556064	99.8	LC547931	100.0	LC549520	100.0	II	II	-	-
				RM9-04	LC556065	99.8	LC547932	100.0	LC549521	100.0	II	II	-	-
				RM9-05	LC556066	99.4	LC547933	100.0	LC549522	100.0	II	II	-	-
Chachoengsao (n = 6)	CS01	13.668917, 101.191042	Pond#5	CCS01	LC556020	99.9	LC547923	100.0	LC549500	100.0	II	II	-	-
	CS03	13.606459, 101.232437	Rice Field#1	CCS03	LC556022	99.8	LC547924	100.0	LC549501	100.0	II	II	-	-
				CCS04	LC556023	100.0	LC547925	100.0	LC549502	100.0	II	II	-	-
				CCS05	LC556024	100.0	LC547926	100.0	LC549503	100.0	II	II	-	-
			Rice Field#2	CCS07	LC556025	99.9	LC547927	100.0	LC549504	100.0	II	II	-	-
			Rice Field#2	CCS08	LC556026	99.9	LC547928	100.0	LC549505	100.0	II	II	-	-
Kanchanaburi (n = 11)	KB01	14.138575, 99.324756	Pond#6	KCB12	LC556043	99.4	-	-	-	-	-	II	-	-
	KB03	14.027293, 99.791957	Rice Field#3	KCB01	LC556032	99.8	LC547939	94.4	LC549506	100.0	III	III	2.490	B
				KCB02	LC556033	99.9	LC547919	99.3	LC549507	100.0	II	II	2.274	A
				KCB03	LC556034	99.7	LC547940	94.3	LC549508	100.0	III	III	2.151	B
				KCB04	LC556035	99.4	LC547941	94.4	LC549509	100.0	III	III	2.187	B
				KCB05	LC556036	99.9	LC547920	99.3	LC549510	100.0	II	II	2.149	A
				KCB06	LC556037	99.7	LC547942	94.4	LC549511	100.0	III	III	-	-
				KCB07	LC556038	100.0	LC547921	99.3	LC549512	100.0	II	II	2.196	A
				KCB08	LC556039	99.9	LC547943	94.4	LC549513	100.0	III	III	2.400	B
				KCB09	LC556040	99.8	LC547944	94.4	LC549514	100.0	III	III	2.187	B
				KCB10	LC556041	99.4	LC547922	99.3	LC549515	100.0	II	II	2.200	A
Ratchaburi (n = 1)	RB01	13.806338, 99.689354	Pond#7	RCB04	LC556056	99.6	LC547936	99.3	LC549518	100.0	II	II	-	-
Trang (n = 2)	TG01	07.534068, 99.618507	Rice Field#4	TRG02	LC556075	99.9	LC547934	100.0	LC549524	100.0	II	II	-	-
Trang (n = 2)	TG01	07.534068, 99.618507	Pond#8	TRG03	LC556076	99.9	LC547935	100.0	LC549525	100.0	II	II	-	-