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Low and high Mg inhibit myoblast fusion. C2C12 cells were treated or not for 24 h with NAC in CM and then cultured in DM for 144 h with different extracellular concentrations of Mg. Caveolin-3 and Myomixer expression was analysed by Western blot. β-actin was used as control of loading. A representative blot (a) and densitometry obtained by ImageLab (b) are shown. * Indicates significance with respect to 1 mM Mg (* p ≤ 0.05; ** p ≤ 0.01).
