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. 2021 Mar 24;10(4):719. doi: 10.3390/cells10040719

Figure 3.

Figure 3

Characteristic of WJ-MSC cultured in different conditions. (A) Population doubling time (PDT) for WJ-MSC cultured in 2D and 3D. 3D cultured WJ-MSC divided more slowly than 2D cultured WJ-MSC during the first passage. However, there are observed no differences in next 3 passages. (B) Cumulative Population Doublings calculation revealed shift between 2D and 3D cultured populations. (C) Senescence process analysis. Number of cells expressing β-galactosidase was significantly higher in 3D cultured WJ-MSC than in initial and 2D cultured populations. (D) Colony forming unit (CFU) frequency reduced significantly during 3D culture of WJ-MSC. Following populations of WJ-MSC were used: from 3rd passage (initial populations), from 7th passage (2D culture) and cultured as spheroids (3D culture). The results are presented as mean values of 3 experiments ± SD. For * < 0.05, ** <0.01, **** < 0.0001.