Figure 6.

Relative gene expression level (fold change, mean ± SD) of neural and pluripotency phenotype in 2D and 3D WJ-MSC cultures. Quantitation of these genes was determined relative to ACTB as a housekeeping gene by quantitative real-time PCR. Changes in gene expression in WJ-MSC cells cultured in 2D and 3D conditions are shown relative to that in cells grown in the initial population. Following populations of WJ-MSC were used: from 3rd passage (initial populations), from 7th passage (2D culture), cultured as spheroids and transferred to 2D (3D culture). Results shown are the mean of 3 independent RNA isolations, for * < 0.05, ** <0.01, **** < 0.0001.