Table 3.
List of primers used for Real Time-Quantitative Polymerase Chain Reaction (RT-qPCR).
| Gene | NCBI Reference Sequence | Product Size | Primer Sequence (5′ -> 3′) |
|---|---|---|---|
| β-Actin | NM_001101.5 | 250 bp | F: CATGTACGTTGCTATCCAGGC R: CTCCTTAATGTCACGCACGAT |
| Nestin1 | NM_006617.2 | 64 bp | F: GGGAAGAGGTGATGGAACCA R: AAGCCCTGAACCCTCTTTGC |
| β-Tubulin III | NM_001197181.2 | 126 bp | F: GGAAGAGGGCGAGATGTACG R: GGGTTTAGACACTGCTGGCT |
| MAP-2 | NM_001375545.1 | 99 bp | F: TTGGTGCCGAGTGAGAAGA R: GTCTGGCAGTGGTTGGTTAA |
| GFAP | NM_001363846.2 | 100 bp | F: CCGACAGCAGGTCCATGT R: GTTGCTGGACGCCATTG |
| Sox2 | NM_003106.4 | 93 bp | F: GTGGAAACTTTTGTCGGAGA R: TTATAATCCGGGTGCTCCTT |
| Rex1 | NM_001304358.2 | 107 bp | F: GCTCCCTTGAATGTTCTTTG R: GCCTGTCATGTACTCAGAAT |
| Nanog | NM_024865.4 | 103 bp | F: GAACCTCAGCTACAAACAGG R: CGTCACACCATTGCTATTCT |
| Oct3/4 (Pou5F1) | NM_001285986.2 | 331 bp | F: CCTGAAGCAGAAGAGGATCACC R: AAAGCGGCAGATGGTCGTTTGG |