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. 2021 Mar 24;10(4):501. doi: 10.3390/antiox10040501

Figure 1.

Figure 1

High-fat diet leads to enhanced liver lipid accumulation in NZO mice. B6 and NZO mice were fed by a standard diet (SD) or a high-fat diet (HFD) until the indicated age of 22 or 39 weeks. Body weight (panel A), liver weight (panel B), triglycerides (TG) (panel C) and plin2 content (panel D) were measured as described in the Methods section. Plin2 (panel D) was determined by immunoblotting and data were normalized towards 22w SD B6 (set as ‘1’). A representative blot is shown in panel F. Representative H&E staining of liver slices to visualize the lipid droplets are shown in panel E. Statistical analysis was performed either by two-way ANOVA comparing NZO mice by diet, unpaired t-test (indicated by *) or one sample t-test (indicated by #), comparing selected samples directly. Statistical significance means: a—versus 22w B6; b—vs. 39w B6; c—vs. 22w Nzo SD; and d—vs. 22w NZO HFD. The data presented are the mean ± standard deviation, n = 5–8.