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. 2021 Mar 24;10(4):501. doi: 10.3390/antiox10040501

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Autophagy-related protein expression in liver of high-fat diet exposed mice. B6 and NZO mice were fed by a standard diet (SD) or a high-fat diet (HFD) as described above. Panel A and panel B shows LC3-I and LC3-II protein amount, whereas p62 (panel D), Atg5 (panel E) and Atg5-Atg12 (panel F) are presented together with respective immunoblots and ponceau staining. Panel C is showing a representative LC3 staining with the relative ponceau staining. All data were normalized towards 22w SD B6 (set as ‘1’). Statistical analysis was performed by one sample t-test (indicated by #) or unpaired t-test (indicated by *), directly comparing two selected samples. Statistical significance means: a—versus 22w B6; b—vs. 39w B6; c—vs. 22w Nzo SD; and d—vs. 22w NZO HFD. The data presented are the mean ± standard deviation, n = 5–8.