. Author manuscript; available in PMC: 2021 Apr 23.

Published in final edited form as: Nat Ecol Evol. 2020 Mar 9;4(4):601–611. doi: 10.1038/s41559-020-1128-3

Table 1.

Environmental conditions used in this study

Environment	Evolution Condition	Measurement Panels				Formulation
Environment	Evolution Condition	Diagnostic	Salt	pH	Temp	Formulation
SC	✓	✓	✓	✓	✓	SC + 2% glu, 30°C
Low Salt	✓		✓			SC + 2% glu + 0.2 M NaCl, 30°C
Med Salt			✓			SC + 2% glu + 0.4 M NaCl, 30°C
High Salt	✓	✓	✓			SC + 2% glu + 0.8 M NaCl, 30°C
pH 3	✓	✓		✓		SC + 2% glu, buffered to pH 3, 30°C
pH 3.8	✓			✓		SC + 2% glu, buffered to pH 3.8, 30°C
pH 6	✓			✓		SC + 2% glu, buffered to pH 6, 30°C
pH 7.3	✓	✓		✓		SC + 2% glu, buffered to pH 7.3, 30°C
Low Temp	✓	✓			✓	SC + 2% glu, 21°C
Med Temp					✓	SC + 2% glu, 34°C
High Temp	✓	✓			✓	SC + 2% glu, 37°C
Low Glu	✓	✓				SC + 0.07% glu, 30°C
Gal	✓	✓				SC + 2% gal, 30°C

SC is synthetic complete medium; glu is glucose; gal is galactose.