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. 2021 Mar 24;9(4):308. doi: 10.3390/vaccines9040308

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Serological cross-reactivity against heterologous viruses. A two-dimensional (2D) virus neutralization test (VNT) was performed using sera from cows vaccinated with the A/SKR/Yeoncheon/2017 (A-1) experimental vaccine and field viruses including A/SKR/Yeoncheon/2017 (A/ASIA/Sea-97-G2, homologous field virus), A/VIT/2013 (A/ASIA/Sea-97), A22/IRQ/24/64 (A/ASIA), A/SKR/Gimpo/2017 (A/ASIA/Sea-97), A/MAY/97 (A/ASIA/Sea-97), and A/SKR/Pocheon/2010 (A/ASIA/Sea-97-G1). The dotted line indicates the cut-off value of 0.3, above which the vaccine is considered to antigenically match with the field virus. Error bars indicate SDs from the mean. Experiments were performed twice independently.