Figure 9.

WW307 triggers the production of ROS in MRSA T144 and E. coli B2. The production of ROS of MRSA T144 (A) and E. coli B2 (B) was determined by 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA, excitation at 488 nm and emission at 525 nm). Before fluorescence assay, probe-labelled cells were incubated with WW307 at 37 °C for 1 h. (C,D) N-acetylcysteine (NAC) supplementation abolished the antibacterial activity of WW307 against MRSA T144 and E. coli B2. In A and B, all data were presented as mean ± SD, and the statistical significance was determined by non-parametric one-way ANOVA (NS, not significant; * p < 0.05, ** p < 0.01, **** p < 0.0001).