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Quantification of Ac-α-tubulin and Ac-histone levels in immunofluorescence assay. DMSO was used as a negative control in each cohort, and Citarinostat was the positive control. Result indicates that TO-317 induces a clear dose-dependent increase in acetylated α-tubulin with minimal cellular accumulation of acetylated histones under the same dosing concentrations. Data reported above are an average of duplicate experiments.
