Figure 6.

Transplant of feces from T5/N4/Rag-TKO mice conferred increased susceptibility to Citrobacter. A, WT C57BL/6 mice (3 weeks old) were subjected to a bowel cleansing followed at 6 h, 1 d, and 2 d, by administration of fecal suspension generated from Rag1-KO (FMT-Rag1-KO) or T5/N4/Rag-TKO mice. B, Fecal C. rodentium CFU over time and cumulative shedding as assessed by area under curve. C–E, Mice were euthanized on day 10 post inoculation to enable bioluminescent assessment of C. rodentium infection (C), spleen weight (D), and stool consistency (E). Data are means ± SEM, n = 5 mice per condition. Abbreviations: SPF, specific-pathogen-free; FMT, fecal microbiota transplantation; CFU, colony forming unit; N4, Nod-like receptor 4; T5, Toll-like receptor 5; TKO, triple knockout; WT, wild type. *P ≤ .05, **P ≤ .01.