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. 2021 Mar 24;9(4):670. doi: 10.3390/microorganisms9040670

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Influence of CRISPRi-mediated repression of genes of the MEP pathway (a) or of carotenogenesis (b) on decaprenoxanthin production by C. glutamicum. In addition, thiE, deoC, and fixB were analyzed. Mean values of biological duplicates are given. Statistical analysis was calculated with ANOVA against all measured decaprenoxanthin production of C. glutamicum MB001 from all Biolector^®flowerplates and is marked by a star (*). As a reference, the decaprenoxanthin production of the empty vector strain C. glutamicum MB001 (pS_dCas9) in biological duplicates of the corresponding experiment is shown. For abbreviations, see Figure 3.