Table 1.
Strains and plasmids used in this study.
| Strain | Characteristics | Reference |
|---|---|---|
| Corynebacterium glutamicum strains | ||
| Wild type (WT) | Wild-type ATCC 13032 | [59] |
| WT ΔaceE | aceE (cg2466) deletion mutant of WT | [60] |
| WT ΔgapA | gapA (cg1791) deletion mutant of WT | [61] |
| WT Δpgi | pgi (cg0973) deletion mutant of WT | [62] |
| WT ΔsdhCAB | sdhCAB (cg0445/0447/0448) deletion mutant of WT | This work |
| WT ΔsugR | sugR (cg2115) deletion mutant of WT | [25] |
| MB001 | Prophage-cured, genome-reduced WT | [57] |
| MB001 ΔcrtR | crtR (cg0725) deletion mutant of MB001 | [63] |
| E. coli strains | ||
| E. coli DH5α | F-thi−1 endA1 hsdr17(r-, m-) supE44 ΔlacU169 (Φ80lacZΔM15) recA1 gyrA96 | [64] |
| Plasmids | ||
| pK19mobsacB | Mobilizable E. coli vector used for the construction of insertion and deletion mutants of C. glutamicum (oriV, sacB, lacZα); KanR | [65] |
| pK19mobsacB-ΔsdhCAB | pK19mobsacB for the deletion of sdhCAB (cg0445/0447/0448); KanR | This work |
| pRG_dCas9 | E. coli/C. glutamicum shuttle clustered regularly interspaced short palindromic repeats interference (CRISPRi) vector, anhydrotetracycline (aTc)- and IPTG-inducible; CmR | [56] |
| piCas | E. coli/B. methanolicus shuttle CRISPRi vector, mannitol-inducible; CmR | [66] |
| pS_dCas9 | pRG_dCas9 carrying the dCas9 handle followed by the terminator from S. pyogenes; CmR | This work |
| pS_dCas9_aceA | pS_dCas9 plasmid carrying the aceA (cg2560) sgRNA | This work |
| pS_dCas9_aceB | pS_dCas9 plasmid carrying the aceB (cg2559) sgRNA | This work |
| pS_dCas9_aceE | pS_dCas9 plasmid carrying the aceE (cg2466) sgRNA | This work |
| pS_dCas9_ackA | pS_dCas9 plasmid carrying the ackA (cg3047) sgRNA | This work |
| pS_dCas9_acn | pS_dCas9 plasmid carrying the acn (cg1737) sgRNA | This work |
| pS_dCas9_crtB | pS_dCas9 plasmid carrying the crtB (cg0721) sgRNA | This work |
| pS_dCas9_crtE | pS_dCas9 plasmid carrying the crtE (cg0723) sgRNA | This work |
| pS_dCas9_crtEb | pS_dCas9 plasmid carrying the crtEb (cg0717) sgRNA | This work |
| pS_dCas9_crtI | pS_dCas9 plasmid carrying the crtI (cg0720) sgRNA | This work |
| pS_dCas9_crtR | pS_dCas9 plasmid carrying the crtR (cg0725) sgRNA | This work |
| pS_dCas9_crtX | pS_dCas9 plasmid carrying the crtX (cg0730) sgRNA | This work |
| pS_dCas9_crtYe | pS_dCas9 plasmid carrying the crtYe (cg0719) sgRNA | This work |
| pS_dCas9_deoC | pS_dCas9 plasmid carrying the deoC (cg0458) sgRNA | This work |
| pS_dCas9_dxr | pS_dCas9 plasmid carrying the dxr (cg2208) sgRNA | This work |
| pS_dCas9_dxs | pS_dCas9 plasmid carrying the dxs (cg2083) sgRNA | This work |
| pS_dCas9_eno | pS_dCas9 plasmid carrying the eno (cg1111) sgRNA | This work |
| pS_dCas9_fba | pS_dCas9 plasmid carrying the fba (cg3068) sgRNA | This work |
| pS_dCas9_fbp | pS_dCas9 plasmid carrying the fbp (cg1157) sgRNA | This work |
| pS_dCas9_fixB | pS_dCas9 plasmid carrying the fixB (cg1386) sgRNA | This work |
| pS_dCas9_fum | pS_dCas9 plasmid carrying the fum (cg1145) sgRNA | This work |
| pS_dCas9_gapA | pS_dCas9 plasmid carrying the gapA (cg1791) sgRNA | This work |
| pS_dCas9_gltA | pS_dCas9 plasmid carrying the gltA (cg0949) sgRNA | This work |
| pS_dCas9_glxR | pS_dCas9 plasmid carrying the glxR (cg0350) sgRNA | This work |
| pS_dCas9_gnd | pS_dCas9 plasmid carrying the gnd (cg1643) sgRNA | This work |
| pS_dCas9_icd | pS_dCas9 plasmid carrying the icd (cg0766) sgRNA | This work |
| pS_dCas9_idsA | pS_dCas9 plasmid carrying the idsA (cg2384) sgRNA | This work |
| pS_dCas9_IIdD | pS_dCas9 plasmid carrying the IIdD (cg3227) sgRNA | This work |
| pS_dCas9_ispE | pS_dCas9 plasmid carrying the ispE (cg1039) sgRNA | This work |
| pS_dCas9_ispF | pS_dCas9 plasmid carrying the ispF (cg2944) sgRNA | This work |
| pS_dCas9_ispG | pS_dCas9 plasmid carrying the ispG (cg2206) sgRNA | This work |
| pS_dCas9_ispH | pS_dCas9 plasmid carrying the ispH (cg1164) sgRNA | This work |
| pS_dCas9_ldh | pS_dCas9 plasmid carrying the ldh (cg3219) sgRNA | This work |
| pS_dCas9_malE | pS_dCas9 plasmid carrying the malE (cg3335) sgRNA | This work |
| pS_dCas9_mdh | pS_dCas9 plasmid carrying the mdh (cg2613) sgRNA | This work |
| pS_dCas9_mmpl | pS_dCas9 plasmid carrying the mmpl (cg0722) sgRNA | This work |
| pS_dCas9_odhA | pS_dCas9 plasmid carrying the odhA (cg1280) sgRNA | This work |
| pS_dCas9_odx | pS_dCas9 plasmid carrying the odx (cg1458) sgRNA | This work |
| pS_dCas9_opcA | pS_dCas9 plasmid carrying the opcA (cg1779) sgRNA | This work |
| pS_dCas9_pck | pS_dCas9 plasmid carrying the pck (cg3169) sgRNA | This work |
| pS_dCas9_pfkA | pS_dCas9 plasmid carrying the pfkA (cg1409) sgRNA | This work |
| pS_dCas9_pgi | pS_dCas9 plasmid carrying the pgi (cg0973) sgRNA | This work |
| pS_dCas9_pgk | pS_dCas9 plasmid carrying the pgk (cg1790) sgRNA | This work |
| pS_dCas9_pgl | pS_dCas9 plasmid carrying the pgl (cg1780) sgRNA | This work |
| pS_dCas9_pgm | pS_dCas9 plasmid carrying the pgm (cg2800) sgRNA | This work |
| pS_dCas9_ppc | pS_dCas9 plasmid carrying the ppc (cg1787) sgRNA | This work |
| pS_dCas9_ppsA | pS_dCas9 plasmid carrying the ppsA (cg0644) sgRNA | This work |
| pS_dCas9_pqo | pS_dCas9 plasmid carrying the pqo (cg2891) sgRNA | This work |
| pS_dCas9_pta | pS_dCas9 plasmid carrying the pta (cg3048) sgRNA | This work |
| pS_dCas9_ptsG | pS_dCas9 plasmid carrying the ptsG (cg1537) sgRNA | This work |
| pS_dCas9_pyc | pS_dCas9 plasmid carrying the pyc (cg0791) sgRNA | This work |
| pS_dCas9_pyk | pS_dCas9 plasmid carrying the pyk (cg2291) sgRNA | This work |
| pS_dCas9_ramB | pS_dCas9 plasmid carrying the ramB (cg0444) sgRNA | This work |
| pS_dCas9_rpe | pS_dCas9 plasmid carrying the rpe (cg1801) sgRNA | This work |
| pS_dCas9_rpi | pS_dCas9 plasmid carrying the rpi (cg2658) sgRNA | This work |
| pS_dCas9_rsdA | pS_dCas9 plasmid carrying the rsdA (cg0697) sgRNA | This work |
| pS_dCas9_rshA | pS_dCas9 plasmid carrying the rshA (cg0877) sgRNA | This work |
| pS_dCas9_sdhA | pS_dCas9 plasmid carrying the sdhA (cg0446) sgRNA | This work |
| pS_dCas9_sdhB | pS_dCas9 plasmid carrying the sdhB (cg0447) sgRNA | This work |
| pS_dCas9_sdhCD | pS_dCas9 plasmid carrying the sdhCD (cg0445) sgRNA | This work |
| pS_dCas9_sigA | pS_dCas9 plasmid carrying the sigA (cg2092) sgRNA | This work |
| pS_dCas9_sigB | pS_dCas9 plasmid carrying the sigB (cg2102) sgRNA | This work |
| pS_dCas9_sigC | pS_dCas9 plasmid carrying the sigC (cg0309) sgRNA | This work |
| pS_dCas9_sigD | pS_dCas9 plasmid carrying the sigD (cg0696) sgRNA | This work |
| pS_dCas9_sigE | pS_dCas9 plasmid carrying the sigE (cg1271) sgRNA | This work |
| pS_dCas9_sigH | pS_dCas9 plasmid carrying the sigH (cg0876) sgRNA | This work |
| pS_dCas9_sigM | pS_dCas9 plasmid carrying the sigM (cg3420) sgRNA | This work |
| pS_dCas9_sucC | pS_dCas9 plasmid carrying the sucC (cg2837) sgRNA | This work |
| pS_dCas9_sucD | pS_dCas9 plasmid carrying the sucD (cg2836) sgRNA | This work |
| pS_dCas9_sugR | pS_dCas9 plasmid carrying the sugR (cg2115) sgRNA | This work |
| pS_dCas9_tal | pS_dCas9 plasmid carrying the tal (cg1776) sgRNA | This work |
| pS_dCas9_thiE | pS_dCas9 plasmid carrying the thiE (cg2236) sgRNA | This work |
| pS_dCas9_tkt | pS_dCas9 plasmid carrying the tkt (cg1774) sgRNA | This work |
| pS_dCas9_tpi | pS_dCas9 plasmid carrying the tpi (cg1789) sgRNA | This work |
| pS_dCas9_zwf | pS_dCas9 plasmid carrying the zwf (cg1778) sgRNA | This work |