Figure 2.

Silencing of miR-150-5p inhibited high glucose-induced podocytes injury. (A) The levels of miR-150-5p in podocytes were determined using quantitative real-time PCR (qRT-PCR) after transfection with anti-miR-150-5p for 48 h. (B) The levels of miR-150-5p in podocytes were determined using qRT-PCR after transfection with anti-miR-150-5p and culture in high glucose for 48 h. (C,D) Quantification and analysis of apoptosis rates using flow cytometry in podocytes after transfection with anti-miR-150-5p and culture in high glucose for 48 h. (E,F) The levels of P-cadherin and zonula occludens-1 (ZO-1) in podocytes were determined using qRT-PCR and Western blot after transfection with anti-miR-150-5p and culture in high glucose for 48 h. Data are expressed as mean ± SEM (n = 3; **p < 0.01, ***p < 0.001).