Table 3. Association between the levels of circDDX10 in granulosa cells (GCs) from human follicular fluids and reproductive outcomes of assisted reproductive technology (ART).
| Parameters | Quartile of the level of circDDX10 in GC from human follicular fluid | P value | |||||||
| Q1 | % | Q2 | % | Q3 | % | Q4 | % | ||
| Oocytes retrieved | |||||||||
| Total | 483 | – | 461 | – | 683 | – | 642 | – | – |
| Mean | 9.9 (7.8–11.9) a | – | 9.4 (7.7–11.1) a | – | 13.4 (11.7–15.) b | – | 12.6 (10.9–14.3) b | – | < 0.01 |
| Normal fertilization rate | |||||||||
| Total | 266/452 | 58.8 | 284/451 | 63.0 | 404/640 | 63.1 | 367/597 | 61.5 | NS |
| IVF cycles | 142/268 | 53.0 | 209/337 | 62.0 | 301/496 | 60.7 | 298/505 | 59.0 | NS |
| ICSI cycles | 124/184 | 67.4 | 75/114 | 65.8 | 103/144 | 71.5 | 69/92 | 75.0 | NS |
| Good quality embryo rate | |||||||||
| Total | 126/266 a | 47.4 | 156/284 a, b | 54.9 | 259/404 b, c | 64.1 | 240/367 c | 65.4 | < 0.01 |
| IVF cycles | 74/142 a | 52.1 | 124/209 a, b | 59.3 | 202/301 b | 67.1 | 196/298 b | 65.8 | < 0.01 |
| ICSI cycles | 52/124 a | 41.9 | 32/75 a, b | 42.7 | 57/103 a, b | 55.3 | 44/69 b | 53.6 | < 0.05 |
| β-hCG positive rate | |||||||||
| Total | 14/23 | 60.9 | 18/25 | 72.0 | 22/28 | 78.6 | 21/25 | 84.0 | NS |
| IVF cycles | 10/17 | 58.8 | 13/18 | 72.2 | 16/21 | 76.2 | 19/21 | 90.5 | NS |
| ICSI cycles | 4/6 | 66.7 | 5/7 | 71.4 | 6/7 | 85.7 | 2/4 | 50.0 | NS |
| Clinical pregnancy rate * | |||||||||
| Total | 9/23 | 39.1 | 11/21 | 52.4 | 19/27 | 70.4 | 18/25 | 72.0 | NS |
| IVF cycles | 8/17 | 47.1 | 8/15 | 53.3 | 13/20 | 65.0 | 16/21 | 76.2 | NS |
| ICSI cycles | 1/6 | 16.7 | 3/6 | 50.0 | 6/7 | 85.7 | 2/4 | 50.0 | NS |
Mean oocytes retrieved are shown as mean (95% CI). a–c, denote the differences between groups (Kruskal-Wallis test and Bonferroni method were used for multiple comparisons, respectively). * A total of five patients’ clinical pregnancy outcomes were unclear. Normal fertilization rates were calculated as: number of 2PN/number of oocytes inseminated. Good quality embryo rates were calculated as: number of good quality embryos/number of 2PN embryos. GC, granulosa cell; ICSI, intracytoplasmic sperm injection; IVF, in vitro fertilization; NS, not statistically significant; Q, quartile; 2PN, two pronuclei.