. 2021 Mar 19;13(7):9780–9800. doi: 10.18632/aging.202732

Table 1. Limiting dilution analyses of tumorigenic lung cancer cells.

Co-implanted cells	no. tumors/ no. injections		Lung cancer-initiating cell frequency (95% CI) (‰)
	cells/injection		Lung cancer-initiating cell frequency (95% CI) (‰)
	20,000	1,000	Estimate	Lower	Upper
A549+LC-MSCs-P1	7/12	3/13	0.117	0.048	0.284
A549+LC-MSCs-P2	5/7	4/7	0.059	0.030	0.118
A549	0/7, 0/6	0/7	-	-	0.011

A549.Luc cells were implanted into female Balb/c nude mice with LC-MSCs or not. The frequency of lung cancer-initiating cells was calculated by limiting dilution analyses (http://bioinf.wehi.edu.au/software/elda/index.html). In all cases, lung cancer-initiating cells were significantly (p<0.05) more frequent in the co-implanted groups. CI: confidence interval.