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. Author manuscript; available in PMC: 2021 Apr 23.
Published in final edited form as: J Mol Biol. 2019 Mar 15;431(10):1940–1955. doi: 10.1016/j.jmb.2019.03.010

Figure 3.

Figure 3.

a) UV thermal melting profile of GQES7-b at 295 nm. Before melting RNA was annealed in the presence of either 100 mM KCl or 100 mM LiCl. b) ES7HS cleavage by mung bean nuclease. ES7 was annealed with or without KCl and with or without PDS. The black arrow indicates cleaved rRNA. c) EMSA of the BG4 antibody with GQES7-a and its non-G-quadruplex-forming mutant mtES7-a, visualized on a native gel. GQES7-a and mtES7-a RNAs were loaded at a constant strand concentration with increasing concentrations of BG4 antibody. The RNA (arrow) is blue and the protein is red.