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. Author manuscript; available in PMC: 2021 Apr 23.
Published in final edited form as: J Mol Biol. 2019 Mar 15;431(10):1940–1955. doi: 10.1016/j.jmb.2019.03.010

Figure 4.

Figure 4.

Dot blots performed with the BG4 antibody on a) GQES7-a and GQES7-b, b) intact ES7HS, c) the negative controls mtES7-b and tRNA, d) the 28S rRNA extracted from HEK293T cells and on e) human 80S ribosomes and f) polysomes purified from HEK293 cells. All samples were incubated in the presence of 50mM KCl and ribosomes and polysomes were further analyzed with or without 10 μM PDS, which stabilizes G-quadruplexes. Samples were loaded onto the membrane in increasing amounts from left to right.