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. 2021 Mar 25;11(4):493. doi: 10.3390/biom11040493

Figure 4.

Figure 4

DEG functions as a full agonist at GlyT1 and GlyT2. (A) Representative original traces of electrophysiological measurements in Xenopus laevis oocytes expressing GlyT1 and GlyT2 individually after superfusion with DEG solution in increasing substance concentration (100 µM, 333 µM, 1 mM, 3.3 mM, 10 mM), sarcosine solution (1 mM) and glycine solution (1 mM) and after superfusion with different glycine solutions (20 µM, 200 µM, 2 mM) additionally containing distinct amounts of DEG (0 mM, 3.3 mM, 10 mM) to create co-application conditions. (B) Dose–response curves of GlyTs showing DEG and sarcosine-induced currents in relation to the maximum observed current induced by 1 mM glycine in comparison to the construct’s glycine dose–response curve (Figure 2B) (GlyT1: DEG n = 8–26, sarcosine n = 19; GlyT2: DEG n = 17, sarcosine n = 16). (C) Relative substance-induced currents of GlyT1 and GlyT2 in relation to the maximum observed current induced by 2 mM glycine + 0 mM DEG solution (GlyT1 n = 13–23; GlyT2 n = 15–31; data shown as boxplots, p < 0.001 (***), one-way ANOVA with Bonferroni post-hoc correction).