Figure 2.

Postmortem stability of genomic epigenetic marks positively associated with transcription. Mice were sacrificed and maintained at room temperature for 6, 24, 48, 72 and 96 h prior to the dissection and brain harvesting. ChiP-qPCR was performed in isolated chromatin for the detection of H3K4me3, H3K27Ac and RNA Pol II with ChIP-grade antibodies. The reactions were performed in triplicate using 25 μg of mouse brain tissue chromatin and 3 μL of H3K4me3 antibody (Active Motif, Cat#39159), 4 μg of H3K27Ac antibody (Active Motif, Cat#39133) or 4 μg of RNA Pol II antibody (Active Motif, Cat#91151). We performed qPCR using two positive control primers that worked well in similar assays (ACTB, GAPDH), as well as a negative control primer pair that amplifies a region in a gene desert on chromosome 6 (Untr6). * p < 0.05 in comparisons indicated by lines.