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. 2021 Mar 25;10(4):259. doi: 10.3390/biology10040259

Figure 2.

Figure 2

Cell cytoskeleton influences the expressions of bone/brain metastasis-related genes. (A, B) The effect of disrupting cell cytoskeleton on bone/brain metastasis-related genes in 231-BoM cells. The cytoskeleton of 231-BoM cells was disrupted by different dosages of Cyto D, Y27632, and Blebbistatin for 24 h. The expressions of bone metastasis-related genes (CXCR4, CTGF, FGF5, ADAMTS1, IMPG1, FST, FYN, OPN, and PTHrP) (A) and brain metastasis-related genes (COX2, ANGPTL4, Serpin B2, LTBP1, PIEZO2, EREG, HBEGF, ITGAV, and ITGB3) (B) were measured by quantitative RT-PCR. (C, D) The effect of stabilizing cell cytoskeleton on bone/brain metastasis-related genes in 231-BrM cells. The cytoskeleton of 231-BrM cells was stabilized by different dosages of Narci and Jas for 24 h. The expressions of bone metastasis-related genes (CXCR4, CTGF, FGF5, ADAMTS1, IMPG1, FST, FYN, OPN, and PTHrP) (C) and brain metastasis-related genes (COX2, ANGPTL4, Serpin B2, LTBP1, PIEZO2, EREG, HBEGF, ITGAV, and ITGB3) (D) were measured by quantitative RT-PCR. n= three independent experiments. The data represent mean ± SEM. * p < 0.05; ** p < 0.01; and *** p < 0.001.