Table 2.

miRNA parameters.

miRNA	miRNA Gene Target	miRNA Accession Number (miRBase)	Nucleotide Sequence of Sense Primer for PCR, 5′-3′	PCR Primers Annealing Temperature, ℃
miR-320a-3p	CDH, CLU	MIMAT0000510	aaaagctgggttgagagggcga	59.5
miR-17-5p	CDH, CLU	MIMAT0000070	caaagtgcttacagtgcaggtag	55
miR-21-5p	CDH, CLU	MIMAT0000076	tagcttatcagactgatgttga	54.6
miR-1323	CDH, CLU	MIMAT0005795	tcaaaactgaggggcattttct	51
miR-25-3p	CDH, CLU	MIMAT0000081	cattgcacttgtctcggtctga	56
miR-138-5p	CDH, CLU	MIMAT0000430	agctggtgttgtgaatcaggccg	54.6
miR-34a-5p	CDH, CLU	MIMAT0000255	tggcagtgtcttagctggttgt	51.3
miR-92a-3p	CDH, CLU	MIMAT0000092	tattgcacttgtcccggcctgt	60
miR-30a-5p	CLU	MIMAT0000087	tgtaaacatcctcgactggaag	54.6
miR-30c-5p	CLU	MIMAT0000244	tgtaaacatcctacactctcagc	49.1
miR-371a-5p	CDH	MIMAT0004687	actcaaactgtgggggcact	54.6
miR-506-3p	CDH	MIMAT0002878	taaggcacccttctgagtaga	51.3
miR-382-5p	endogenous control for PCR	MIMAT0000737	gaagttgttcgtggtggattcg	49.1